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| Item Type: | Preprint |
|---|---|
| Title: | Massively parallel clonal analysis using CRISPR/Cas9 induced genetic scars |
| Creators Name: | Junker, J.P., Spanjaard, B., Peterson-Maduro, J., Alemany, A., Hu, B., Florescu, M. and van Oudenaarden, A. |
| Abstract: | A key goal of developmental biology is to understand how a single cell transforms into a full-grown organism consisting of many cells. Although impressive progress has been made in lineage tracing using imaging approaches, analysis of vertebrate lineage trees has mostly been limited to relatively small subsets of cells. Here we present scartrace, a strategy for massively parallel clonal analysis based on Cas9 induced genetic scars in the zebrafish. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 056499 |
| Date: | 4 January 2017 |
| Official Publication: | https://doi.org/10.1101/056499 |
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