Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

C/EBP-induced transdifferentiation reveals granulocyte-macrophage precursor-like plasticity of B cells

[thumbnail of 16283oa.pdf]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
10MB

Item Type:Article
Title:C/EBP-induced transdifferentiation reveals granulocyte-macrophage precursor-like plasticity of B cells
Creators Name:Cirovic, B., Schönheit, J., Kowenz-Leutz, E., Ivanovska, J., Klement, C., Pronina, N., Bégay, V. and Leutz, A.
Abstract:The lymphoid-myeloid transdifferentiation potentials of members of the C/EBP family (C/EBP{alpha}, {beta}, {delta}, and {epsilon}) were compared in v-Abl-immortalized primary B cells. Conversion of B cells to macrophages was readily induced by the ectopic expression of any C/EBP, and enhanced by endogenous C/EBP{alpha} and {beta} activation. High transgene expression of C/EBP{beta} or C/EBP{epsilon}, but not of C/EBP{alpha} or C/EBP{delta}, also induced the formation of granulocytes. Granulocytes and macrophages emerged in a mutually exclusive manner. C/EBP{beta}-expressing B cells produced granulocyte-macrophage progenitor (GMP)-like progenitors when subjected to selective pressure to eliminate lymphoid cells. The GMP-like progenitors remained self-renewing and cytokine-independent, and continuously produced macrophages and granulocytes. In addition to their suitability to study myelomonocytic lineage bifurcation, lineage-switched GMP-like progenitors could reflect the features of the lympho-myeloid lineage switch observed in leukemic progression.
Keywords:Hematopoiesis, Leukemia, Differentiation, Stem Cell, Progenitor, Lineage Switch, Animals, Mice
Source:Stem Cell Reports
ISSN:2213-6711
Publisher:Cell Press / Elsevier
Volume:14
Number:8
Page Range:346-359
Date:14 February 2017
Official Publication:https://doi.org/10.1016/j.stemcr.2016.12.015
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library