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| Item Type: | Article |
|---|---|
| Title: | Combining transcription factor binding affinities with open-chromatin data for accurate gene expression prediction |
| Creators Name: | Schmidt, F., Gasparoni, N., Gasparoni, G., Gianmoena, K., Cadenas, C., Polansky, J.K., Ebert, P., Nordstroem, K., Barann, M., Sinha, A., Froehler, S., Xiong, J., Dehghani Amirabad, A., Behjati Ardakani, F., Hutter, B., Zipprich, G., Felder, B., Eils, J., Brors, B., Chen, W., Hengstler, J.G., Hamann, A., Lengauer, T., Rosenstiel, P., Walter, J. and Schulz, M.H. |
| Abstract: | The binding and contribution of transcription factors (TF) to cell specific gene expression is often deduced from open-chromatin measurements to avoid costly TF ChIP-seq assays. Thus, it is important to develop computational methods for accurate TF binding prediction in open-chromatin regions (OCRs). Here, we report a novel segmentation-based method, TEPIC, to predict TF binding by combining sets of OCRs with position weight matrices. TEPIC can be applied to various open-chromatin data, e.g. DNaseI-seq and NOMe-seq. Additionally, Histone-Marks (HMs) can be used to identify candidate TF binding sites. TEPIC computes TF affinities and uses open-chromatin/HM signal intensity as quantitative measures of TF binding strength. Using machine learning, we find low affinity binding sites to improve our ability to explain gene expression variability compared to the standard presence/absence classification of binding sites. Further, we show that both footprints and peaks capture essential TF binding events and lead to a good prediction performance. In our application, gene-based scores computed by TEPIC with one open-chromatin assay nearly reach the quality of several TF ChIP-seq data sets. Finally, these scores correctly predict known transcriptional regulators as illustrated by the application to novel DNaseI-seq and NOMe-seq data for primary human hepatocytes and CD4+ T-cells, respectively. |
| Keywords: | Algorithms, Binding Sites, CD4-Positive T-Lymphocytes, Cell Line, Chromatin, Chromatin Assembly and Disassembly, DNA, Gene Expression Regulation, Hep G2 Cells, Hepatocytes, Histones, Human Embryonic Stem Cells, K562 Cells, Machine Learning, Organ Specificity, Primary Cell Culture, Principal Component Analysis, Protein Binding, Transcription Factors, Tumor Cell Line |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 45 |
| Number: | 1 |
| Page Range: | 54-66 |
| Date: | 9 January 2017 |
| Official Publication: | https://doi.org/10.1093/nar/gkw1061 |
| PubMed: | View item in PubMed |
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