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E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton

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Item Type:Article
Title:E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton
Creators Name:Huelsken, J., Birchmeier, W. and Behrens, J.
Abstract:beta-Catenin is involved in the formation of adherens junctions of mammalian epithelia. It interacts with the cell adhesion molecule E-cadherin and also with the tumor suppressor gene product APC, and the Drosophila homologue of beta-catenin, armadillo, mediates morphogenetic signals. We demonstrate here that E-cadherin and APC directly compete for binding to the internal, armadillo-like repeats of beta-catenin; the NH2-terminal domain of beta-catenin mediates the interaction of the alternative E-cadherin and APC complexes to the cytoskeleton by binding to alpha-catenin. Plakoglobin (gamma-catenin), which is structurally related to beta-catenin, mediates identical interactions. We thus show that the APC tumor suppressor gene product forms strikingly similar associations as found in cell junctions and suggest that beta-catenin and plakoglobin are central regulators of cell adhesion, cytoskeletal interaction, and tumor suppression.
Keywords:Adenomatous Polyposis Coli Protein, alpha Catenin, Amino Acid Sequence, Base Sequence, beta Catenin, Cadherins, Cultured Cells, Cytoskeletal Proteins, Cytoskeleton, Desmoplakins, Fluorescent Antibody Technique, gamma Catenin, Intercellular Junctions, Molecular Models, Molecular Sequence Data, Precipitin Tests, Protein Binding, Recombinant Proteins, Site-Directed Mutagenesis, Trans-Activators
Source:Journal of Cell Biology
Publisher:Rockefeller University Press
Number:6 Pt. 2
Page Range:2061-2069
Date:December 1994
Additional Information:Copyright (c) 1994 by The Rockefeller University Press
Official Publication:http://www.jcb.org/cgi/content/abstract/127/6/2061
PubMed:View item in PubMed

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