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| Item Type: | Article |
|---|---|
| Title: | TLR2 controls random motility, while TLR7 regulates chemotaxis of microglial cells via distinct pathways |
| Creators Name: | Ifuku, M., Buonfiglioli, A., Jordan, P., Lehnardt, S. and Kettenmann, H. |
| Abstract: | Microglial cells are the pathologic sensor of the brain, and any pathologic event triggers microglial activation, which involves migration of these cells to a lesion site. Employing different migration assays, we show that ligands for toll-like receptor (TLR) 2 stimulate random motility, while TLR7 ligands are chemoattractants. The subtype specificity of the TLR ligands was verified by using different TLR-deficient (TLRKO) mouse lines. PI3K and Rac inhibition impairs both TLR2- and TLR7-stimulated microglial migration. In contrast, Akt phosphorylation is only required for the TLR2-, but not for the TLR7-stimulated pathway. Interestingly, P2Y12 receptor signaling is involved in the TLR2 activation-induced microglial migration but not TLR7. Furthermore, TLR7 mRNA expression is down-regulated by TLR2 and TLR7 activation. We conclude that TLRs control the migratory behavior of microglia in a distinct manner. |
| Keywords: | Toll-Like Receptor, Migration, Chemotaxis, PI3K, Akt, Rac, Animals, Mice |
| Source: | Brain Behavior and Immunity |
| ISSN: | 0889-1591 |
| Publisher: | Elsevier / Academic Press |
| Volume: | 58 |
| Page Range: | 338-347 |
| Date: | November 2016 |
| Official Publication: | https://doi.org/10.1016/j.bbi.2016.08.003 |
| PubMed: | View item in PubMed |
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