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| Item Type: | Article |
|---|---|
| Title: | CCL4 as an adjuvant for DNA vaccination in a Her2/neu mouse tumor model |
| Creators Name: | Nguyen-Hoai, T., Pham-Duc, M., Gries, M., Dörken, B., Pezzutto, A. and Westermann, J. |
| Abstract: | Chemokines are key regulators of both innate and adaptive immune responses. CCL4 (macrophage inflammatory protein-1{beta}, MIP-1{beta}) is a CC chemokine that has a broad spectrum of target cells including immature dendritic cells, which express the cognate receptor CCR5. We asked whether a plasmid encoding CCL4 is able to improve tumor protection and immune responses in a Her2/neu+ mouse tumor model. Balb/c mice were immunized twice intramuscularly with plasmid DNA on days 1 and 15. On day 25, a tumor challenge was performed with 2 × 10(5) syngeneic Her2/neu+ D2F2/E2 tumor cells. Different groups of mice were vaccinated with pDNA(Her2/neu) plus pDNA(CCL4), pDNA(Her2/neu), pDNA(CCL4) or mock vector alone. Our results show that CCL4 is able to (i) improve tumor protection and (ii) augment a TH1-polarized immune response against Her2/neu. Although Her2/neu-specific humoral and T-cell immune responses were comparable with that induced in previous studies using CCL19 or CCL21 as adjuvants, tumor protection conferred by CCL4 was inferior. Whether this is due to a different spectrum of (innate) immune cells, remains to be clarified. However, combination of CCL19/21 with CCL4 might be a reasonable approach in the future, particularly for DNA vaccination in Her2/neu+ breast cancer in the situation of minimal residual disease. |
| Keywords: | Animal Disease Models, Cancer Vaccines, Cell Line, Chemokine CCL4, DNA Vaccines, ErbB-2 Receptor, Experimental Mammary Neoplasms, Gene Expression, Gene Order, Immunization, Immunologic Adjuvants, Plasmids, Tumor Burden, Animals, Mice |
| Source: | Cancer Gene Therapy |
| ISSN: | 0929-1903 |
| Publisher: | Nature Publishing Group |
| Volume: | 23 |
| Number: | 6 |
| Page Range: | 162-167 |
| Date: | June 2016 |
| Official Publication: | https://doi.org/10.1038/cgt.2016.9 |
| PubMed: | View item in PubMed |
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