![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
Preview |
PDF (Original article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1MB |
Preview |
PDF (Supplementary information)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB |
| Item Type: | Article |
|---|---|
| Title: | LHX2 is a direct NF-κB target gene that promotes primary hair follicle placode down-growth |
| Creators Name: | Tomann, P., Paus, R., Millar, S.E., Scheidereit, C. and Schmidt-Ullrich, R. |
| Abstract: | In the epidermis of mice lacking transcription factor nuclear factor-kappa B (NF-{kappa}B) activity, primary hair follicle (HF) pre-placode formation is initiated without progression to proper placodes. NF-{kappa}B modulates WNT and SHH signaling at early stages of HF development, but this does not fully account for the phenotypes observed upon NF-{kappa}B inhibition. To identify additional NF-{kappa}B target genes, we developed a novel method to isolate and transcriptionally profile primary HF placodes with active NF-{kappa}B signaling. In parallel, we compared gene expression at the same developmental stage in NF-{kappa}B-deficient embryos and controls. This uncovered novel NF-{kappa}B target genes with potential roles in priming HF placodes for down-growth. Importantly, we identify Lhx2 (encoding a LIM/homeobox transcription factor) as a direct NF-{kappa}B target gene, loss of which replicates a subset of phenotypes seen in NF-{kappa}B-deficient embryos. Lhx2 and Tgfb2 knockout embryos exhibit very similar abnormalities in HF development, including failure of the E-cadherin suppression required for follicle down-growth. We show that TGF{beta}2 signaling is impaired in NF-{kappa}B-deficient and Lhx2 knockout embryos and that exogenous TGF{beta}2 rescues the HF phenotypes in Lhx2 knockout skin explants, indicating that it operates downstream of LHX2. These findings identify a novel NF-{kappa}B/LHX2/TGF{beta}2 signaling axis that is crucial for primary HF morphogenesis, which may also function more broadly in development and disease. |
| Keywords: | NF-{kappa}B, LHX2, Hair Follicle, TGF{beta}2, Cell Migration, E-Cadherin, EDA-A1, EDAR, Embryo, Placode, Stem Cell, Animals, Mice |
| Source: | Development |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Volume: | 143 |
| Number: | 9 |
| Page Range: | 1512-1522 |
| Date: | 1 May 2016 |
| Official Publication: | https://doi.org/10.1242/dev.130898 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
