![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of 15467oa.pdf]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB |
| Item Type: | Article |
|---|---|
| Title: | Ryanodine receptor activation induces long-term plasticity of spine calcium dynamics |
| Creators Name: | Johenning, F.W., Theis, A.K., Pannasch, U., Rückl, M., Rüdiger, S. and Schmitz, D. |
| Abstract: | A key feature of signalling in dendritic spines is the synapse-specific transduction of short electrical signals into biochemical responses. Ca(2+) is a major upstream effector in this transduction cascade, serving both as a depolarising electrical charge carrier at the membrane and an intracellular second messenger. Upon action potential firing, the majority of spines are subject to global back-propagating action potential (bAP) Ca(2+) transients. These transients translate neuronal suprathreshold activation into intracellular biochemical events. Using a combination of electrophysiology, two-photon Ca(2+) imaging, and modelling, we demonstrate that bAPs are electrochemically coupled to Ca2+ release from intracellular stores via ryanodine receptors (RyRs). We describe a new function mediated by spine RyRs: the activity-dependent long-term enhancement of the bAP-Ca(2+) transient. Spines regulate bAP Ca(2+) influx independent of each other, as bAP-Ca(2+) transient enhancement is compartmentalized and independent of the dendritic Ca(2+) transient. Furthermore, this functional state change depends exclusively on bAPs travelling antidromically into dendrites and spines. Induction, but not expression, of bAP-Ca(2+) transient enhancement is a spine-specific function of the RyR. We demonstrate that RyRs can form specific Ca(2+) signalling nanodomains within single spines. Functionally, RyR mediated Ca(2+) release in these nanodomains induces a new form of Ca(2+) transient plasticity that constitutes a spine specific storage mechanism of neuronal suprathreshold activity patterns. |
| Keywords: | Action Potentials, Calcium, Dendritic Spines, Entorhinal Cortex, Hippocampal CA1 Region, Neuronal Plasticity, Patch-Clamp Techniques, Ryanodine Receptor Calcium Release Channel, Wistar Rats, Animals, Rats |
| Source: | PLoS Biology |
| ISSN: | 1545-7885 |
| Publisher: | Public Library of Science |
| Volume: | 13 |
| Number: | 6 |
| Page Range: | e1002181 |
| Date: | 22 June 2015 |
| Official Publication: | https://doi.org/10.1371/journal.pbio.1002181 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
