Synaptic network activity induces neuronal differentiation of adult hippocampal precursor cells through BDNF signaling

Babu, H.; Ramirez-Rodriguez, G.; Fabel, K.; Bischofberger, J.; Kempermann, G.

Synaptic network activity induces neuronal differentiation of adult hippocampal precursor cells through BDNF signaling

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Item Type:	Article
Title:	Synaptic network activity induces neuronal differentiation of adult hippocampal precursor cells through BDNF signaling
Creators Name:	Babu, H., Ramirez-Rodriguez, G., Fabel, K., Bischofberger, J. and Kempermann, G.
Abstract:	Adult hippocampal neurogenesis is regulated by activity. But how do neural precursor cells in the hippocampus respond to surrounding network activity and translate increased neural activity into a developmental program? Here we show that long-term potentiation (LTP)-like synaptic activity within a cellular network of mature hippocampal neurons promotes neuronal differentiation of newly generated cells. In co-cultures of precursor cells with primary hippocampal neurons, LTP-like synaptic plasticity induced by addition of glycine in Mg(2+)-free media for 5 min, produced synchronous network activity and subsequently increased synaptic strength between neurons. Furthermore, this synchronous network activity led to a significant increase in neuronal differentiation from the co-cultured neural precursor cells. When applied directly to precursor cells, glycine- and Mg(2+)-free solution did not induce neuronal differentiation. Synaptic plasticity-induced neuronal differentiation of precursor cells was observed in the presence of GABAergic neurotransmission blockers but was dependent on NMDA-mediated Ca(2+) influx. Most importantly, neuronal differentiation required the release of brain-derived neurotrophic factor (BDNF) from the underlying substrate hippocampal neurons as well as TrkB receptor phosphorylation in precursor cells. This suggests that activity-dependent stem cell differentiation within the hippocampal network is mediated via synaptically evoked BDNF signaling.
Keywords:	Hippocampus, Precursor Cell, Adult Neurogenesis, Long-Term Potentiation, Neurotrophins, Network Oscillation
Source:	Frontiers in Neuroscience
ISSN:	1662-453X
Publisher:	Frontiers Media SA
Volume:	3
Page Range:	49
Date:	30 September 2009
Official Publication:	https://doi.org/10.3389/neuro.22.001.2009
PubMed:	View item in PubMed

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