![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of Article]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF (Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB |
|
PDF (Supplemental information)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
8MB |
| Item Type: | Article |
|---|---|
| Title: | Knockout of the PKN family of Rho effector kinases reveals a non-redundant role for PKN2 in developmental mesoderm expansion |
| Creators Name: | Quétier, I., Marshall, J.J.T., Spencer-Dene, B., Lachmann, S., Casamassima, A., Franco, C., Escuin, S., Worrall, J.T., Baskaran, P., Rajeeve, V., Howell, M., Copp, A.J., Stamp, G., Rosewell, I., Cutillas, P., Gerhardt, H., Parker, P.J. and Cameron, A.J.M. |
| Abstract: | In animals, the protein kinase C (PKC) family has expanded into diversely regulated subgroups, including the Rho family-responsive PKN kinases. Here, we describe knockouts of all three mouse PKN isoforms and reveal that PKN2 loss results in lethality at embryonic day 10 (E10), with associated cardiovascular and morphogenetic defects. The cardiovascular phenotype was not recapitulated by conditional deletion of PKN2 in endothelial cells or the developing heart. In contrast, inducible systemic deletion of PKN2 after E7 provoked collapse of the embryonic mesoderm. Furthermore, mouse embryonic fibroblasts, which arise from the embryonic mesoderm, depend on PKN2 for proliferation and motility. These cellular defects are reflected in vivo as dependence on PKN2 for mesoderm proliferation and neural crest migration. We conclude that failure of the mesoderm to expand in the absence of PKN2 compromises cardiovascular integrity and development, resulting in lethality. |
| Keywords: | Antineoplastic Agents, Apoptosis, Cell Line, Cell Movement, Cell Proliferation, Embryonic Development, Heart, Inbred C57BL Mice, Knockout Mice, Mammalian Embryo, Mesoderm, Myocardium, Protein Kinase C, Reporter Genes, Scanning Electron Microscopy, Tamoxifen, Animals, Mice |
| Source: | Cell Reports |
| ISSN: | 2211-1247 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 14 |
| Number: | 3 |
| Page Range: | 440-448 |
| Date: | 26 January 2016 |
| Official Publication: | https://doi.org/10.1016/j.celrep.2015.12.049 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
