Identification of signature volatiles to discriminate Candida albicans, glabrata, krusei and tropicalis using gas chromatography and mass spectrometry

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Item Type:	Article
Title:	Identification of signature volatiles to discriminate Candida albicans, glabrata, krusei and tropicalis using gas chromatography and mass spectrometry
Creators Name:	Hertel, M., Hartwig, S., Schuette, E., Gillissen, B., Preissner, R., Schmidt-Westhausen, A.M., Paris, S., Kastner, I. and Preissner, S.
Abstract:	Oral candidiasis is the most frequent fungal infection of the oral cavity. Clinical diagnoses require mycological confirmation, which is time-consuming in case of culture testing. The aim of the study was to identify signature volatiles to develop a chairside breath test to diagnose oral candidiasis. Headspaces above Candida albicans, glabrata, tropicalis, krusei cultures, and growth media as control were analysed after eight and 24 h using offline gas chromatography and mass spectrometry. The identification of signature volatiles was assisted using various microbial databases. Retrieved volatile patterns enabled Candida species discrimination in vitro. For C. albicans 3-methyl-2-butanone and styrene and for C. krusei a combination of p-xylene, 2-octanone, 2-heptanone and n-butyl acetate were found to be specific. 1-hexanol was found in C. tropicalis, but is emitted by a variety of other microorganisms. C. glabrata was characterised through the absence of these volatiles. The development of a breath test is a promising approach in confirming suspicions of oral candidiasis. To confirm the retrieved results in vivo, breath tests in affected and healthy subjects have to be performed.
Keywords:	Candida Spp, Oral Candidiasis, Volatile Organic Compounds, Breath Test
Source:	Mycoses
ISSN:	0933-7407
Publisher:	Wiley-Blackwell
Volume:	59
Number:	2
Page Range:	117-126
Date:	February 2016
Official Publication:	https://doi.org/10.1111/myc.12442
PubMed:	View item in PubMed

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