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| Item Type: | Review |
|---|---|
| Title: | Proteomic techniques to probe the ubiquitin landscape |
| Creators Name: | Beaudette, P., Popp, O. and Dittmar, G. |
| Abstract: | Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct signals, either by adding a single ubiquitin or forming a chain of ubiquitins on the target protein. The enzymatic cascade associated with the cellular process determines the nature of the modification. Numerous efforts have been made for the identification of ubiquitin acceptor sites in the target proteins using genetic, biochemical or mass-spectrometry based proteomic methods, such as affinity-based enrichment of ubiquitinated proteins, and antibody-based enrichment of modified peptides. Modern instrumentation enables quantitative mass-spectrometry strategies to identify and characterize hundreds of ubiquitin substrates in a single analysis making it the dominant method for ubiquitin site detection. Characterization of the inter-ubiquitin connectivity in ubiquitin polymers has also moved into focus, with the field of targeted proteomics techniques proving invaluable for identifying and quantifying linkage types found in such polyubiquitin chains. This review seeks to provide an overview of the many mass-spectrometry based proteomics techniques available for exploring this dynamic field. |
| Keywords: | Mass Spectrometry, Proteomics, Ubiquitin, Ubiquitin-Chain, Ubiquitination, Animals |
| Source: | Proteomics |
| ISSN: | 1615-9853 |
| Publisher: | Wiley |
| Volume: | 16 |
| Number: | 2 |
| Page Range: | 273-287 |
| Date: | January 2016 |
| Official Publication: | https://doi.org/10.1002/pmic.201500290 |
| PubMed: | View item in PubMed |
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