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| Item Type: | Article |
|---|---|
| Title: | Role of CYP eicosanoids in the regulation of pharyngeal pumping and food uptake in Caenorhabditis elegans |
| Creators Name: | Zhou, Y., Falck, J.R., Rothe, M., Schunck, W.H. and Menzel, R. |
| Abstract: | Cytochrome P450 (CYP)-dependent eicosanoids comprise epoxy- and hydroxy-metabolites of long-chain PUFAs (LC-PUFAs). In mammals, CYP eicosanoids contribute to the regulation of cardiovascular and renal function. Caenorhabditis elegans produces a large set of CYP eicosanoids; however, their role in worm's physiology is widely unknown. Mutant strains deficient in LC-PUFA/eicosanoid biosynthesis displayed reduced pharyngeal pumping frequencies. This impairment was rescued by long-term eicosapentaenoic and/or arachidonic acid supplementation, but not with a nonmetabolizable LC-PUFA analog. Short-term treatment with 17,18-epoxyeicosatetraenoic acid (17,18-EEQ), the most abundant CYP eicosanoid in C. elegans, was as effective as long-term LC-PUFA supplementation in the mutant strains. In contrast, 20-HETE caused decreased pumping frequencies. The opposite effects of 17,18-EEQ and 20-HETE were mirrored by the actions of neurohormones. 17,18-EEQ mimicked the stimulating effect of serotonin when added to starved worms, whereas 20-HETE shared the inhibitory effect of octopamine in the presence of abundant food. In wild-type worms, serotonin increased free 17,18-EEQ levels, whereas octopamine selectively induced the synthesis of hydroxy-metabolites. These results suggest that CYP eicosanoids may serve as second messengers in the regulation of pharyngeal pumping and food uptake in C. elegans. |
| Keywords: | Cytochrome P45, Fatty Acid, Omega-3 Fatty Acids, Lipidomics, Pharynx, 17,18-EEQ, Animals, Caenorhabditis elegans |
| Source: | Journal of Lipid Research |
| ISSN: | 0022-2275 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 56 |
| Number: | 11 |
| Page Range: | 2110-2123 |
| Date: | November 2015 |
| Official Publication: | https://doi.org/10.1194/jlr.M061887 |
| PubMed: | View item in PubMed |
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