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Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions

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Item Type:Review
Title:Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions
Creators Name:Meyer, K. and Selbach, M.
Abstract:While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of protein-protein interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-scale protein interaction networks. However, most of these approaches are not quantitative and therefore cannot reveal how perturbations affect the network. Here, we illustrate how a clever combination of quantitative mass spectrometry with different biochemical methods provides a rich toolkit to study different aspects of PPIs including topology, subunit stoichiometry, and dynamic behavior.
Keywords:Mass Spectrometry Based Proteomics, Quantitative Proteomics, Protein-Protein Interaction, Stoichiometry, Cross-Linking
Source:Frontiers in Genetics
ISSN:1664-8021
Publisher:Frontiers Media SA
Volume:6
Page Range:237
Date:July 2015
Official Publication:https://doi.org/10.3389/fgene.2015.00237
PubMed:View item in PubMed

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