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| Item Type: | Review |
|---|---|
| Title: | Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions |
| Creators Name: | Meyer, K. and Selbach, M. |
| Abstract: | While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of protein-protein interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-scale protein interaction networks. However, most of these approaches are not quantitative and therefore cannot reveal how perturbations affect the network. Here, we illustrate how a clever combination of quantitative mass spectrometry with different biochemical methods provides a rich toolkit to study different aspects of PPIs including topology, subunit stoichiometry, and dynamic behavior. |
| Keywords: | Mass Spectrometry Based Proteomics, Quantitative Proteomics, Protein-Protein Interaction, Stoichiometry, Cross-Linking |
| Source: | Frontiers in Genetics |
| ISSN: | 1664-8021 |
| Publisher: | Frontiers Media SA |
| Volume: | 6 |
| Page Range: | 237 |
| Date: | July 2015 |
| Official Publication: | https://doi.org/10.3389/fgene.2015.00237 |
| PubMed: | View item in PubMed |
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