Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions

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Item Type:	Review
Title:	Quantitative affinity purification mass spectrometry: a versatile technology to study protein-protein interactions
Creators Name:	Meyer, K. and Selbach, M.
Abstract:	While the genomic revolution has dramatically accelerated the discovery of disease-associated genes, the functional characterization of the corresponding proteins lags behind. Most proteins fulfill their tasks in complexes with other proteins, and analysis of protein-protein interactions (PPIs) can therefore provide insights into protein function. Several methods can be used to generate large-scale protein interaction networks. However, most of these approaches are not quantitative and therefore cannot reveal how perturbations affect the network. Here, we illustrate how a clever combination of quantitative mass spectrometry with different biochemical methods provides a rich toolkit to study different aspects of PPIs including topology, subunit stoichiometry, and dynamic behavior.
Keywords:	Mass Spectrometry Based Proteomics, Quantitative Proteomics, Protein-Protein Interaction, Stoichiometry, Cross-Linking
Source:	Frontiers in Genetics
ISSN:	1664-8021
Publisher:	Frontiers Media SA
Volume:	6
Page Range:	237
Date:	July 2015
Official Publication:	https://doi.org/10.3389/fgene.2015.00237
PubMed:	View item in PubMed

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