A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional

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Item Type:	Article
Title:	A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional
Creators Name:	Tardiff, D.F., Lacadie, S.A. and Rosbash, M.
Abstract:	Recent ChIP experiments indicate that spliceosome assembly and splicing can occur cotranscriptionally in S. cerevisiae. However, only a few genes have been examined, and all have long second exons. To extend these studies, we analyzed intron-containing genes with different second exon lengths by using ChIP as well as whole-genome tiling arrays (ChIP-CHIP). The data indicate that U1 snRNP recruitment is independent of exon length. Recursive splicing constructs, which uncouple U1 recruitment from transcription, suggest that cotranscriptional U1 recruitment contributes to optimal splicing efficiency. In contrast, U2 snRNP recruitment, as well as cotranscriptional splicing, is deficient on short second exon genes. We estimate that > or =90% of endogenous yeast splicing is posttranscriptional, consistent with an analysis of posttranscriptional snRNP-associated pre-mRNA.
Keywords:	Actins, Base Sequence, Chromatin Immunoprecipitation, Exons, Fungal Genome, Fungal RNA, Molecular Sequence Data, Post-Transcriptional RNA Processing, RNA Precursors, RNA Splicing, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Spliceosomes, U1 Small Nuclear Ribonucleoprotein
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press
Volume:	24
Number:	6
Page Range:	917-929
Date:	28 December 2006
Additional Information:	Erratum in: Mol Cell 7 August 2024 [in Press]
Official Publication:	https://doi.org/10.1016/j.molcel.2006.12.002
PubMed:	View item in PubMed

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