Global analysis of nascent RNA reveals transcriptional pausing in terminal exons

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Item Type:	Article
Title:	Global analysis of nascent RNA reveals transcriptional pausing in terminal exons
Creators Name:	Carrillo Oesterreich, F., Preibisch, S. and Neugebauer, K.M.
Abstract:	Pre-mRNA splicing is catalyzed by the spliceosome, which can assemble on pre-mRNA cotranscriptionally. However, whether splicing generally occurs during transcription has not been addressed. Indeed, splicing catalysis is expected to occur posttranscriptionally in yeast, where the shortness of terminal exons should leave insufficient time for splicing. Here, we isolate endogenous S. cerevisiae nascent RNA and determine gene-specific splicing efficiencies and transcription profiles, using high-density tiling microarrays. Surprisingly, we find that splicing occurs cotranscriptionally for the majority of intron-containing genes. Analysis of transcription profiles reveals Pol II pausing within the terminal exons of these genes. Intronless and inefficiently spliced genes lack this pause. In silico simulations of transcription and splicing kinetics confirm that this pausing event provides sufficient time for splicing before termination. The discovery of terminal exon pausing demonstrates functional coupling of transcription and splicing near gene ends.
Keywords:	Chromatin, Exons, Fungal Genes, Fungal RNA, Genetic Transcription, Oligonucleotide Array Sequence Analysis, Proteomics, RNA Polymerase II, RNA Precursors, RNA Splicing, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Subcellular Fractions
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press
Volume:	40
Number:	4
Page Range:	571-581
Date:	24 November 2010
Official Publication:	https://doi.org/10.1016/j.molcel.2010.11.004
PubMed:	View item in PubMed

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