![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of Date Supplement]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF (Date Supplement)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
6MB |
|
PDF (Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
15MB |
| Item Type: | Article |
|---|---|
| Title: | Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum |
| Creators Name: | Staudacher, J.J., Naarmann-de Vries, I.S., Ujvari, S.J., Klinger, B., Kasim, M., Benko, E., Ostareck-Lederer, A., Ostareck, D.H., Bondke Persson, A., Lorenzen, S., Meier, J.C., Blüthgen, N., Persson, P.B., Henrion-Caude, A., Mrowka, R. and Faehling, M. |
| Abstract: | Protein synthesis is a primary energy-consuming process in the cell. Therefore, under hypoxic conditions, rapid inhibition of global mRNA translation represents a major protective strategy to maintain energy metabolism. How some mRNAs, especially those that encode crucial survival factors, continue to be efficiently translated in hypoxia is not completely understood. By comparing specific transcript levels in ribonucleoprotein complexes, cytoplasmic polysomes and endoplasmic reticulum (ER)-bound ribosomes, we show that the synthesis of proteins encoded by hypoxia marker genes is favoured at the ER in hypoxia. Gene expression profiling revealed that transcripts particularly increased by the HIF-1 transcription factor network show hypoxia-induced enrichment at the ER. We found that mRNAs favourably translated at the ER have higher conservation scores for both the 5'- and 3'-untranslated regions (UTRs) and contain less upstream initiation codons (uAUGs), indicating the significance of these sequence elements for sustained mRNA translation under hypoxic conditions. Furthermore, we found enrichment of specific cis-elements in mRNA 5'- as well as 3'-UTRs that mediate transcript localization to the ER in hypoxia. We conclude that transcriptome partitioning between the cytoplasm and the ER permits selective mRNA translation under conditions of energy shortage. |
| Keywords: | {alpha} Subunit Hypoxia-Inducible Factor 1 , Cell Hypoxia, Cell Line, Cytoplasm, Endoplasmic Reticulum, Gene Expression, Genetic Markers, Initiator Codon, Procollagen-Proline Dioxygenase, Protein Biosynthesis, Protein Disulfide-Isomerases, Messenger RNA, Ribosomes, Transcriptome |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 43 |
| Number: | 6 |
| Page Range: | 3219-3236 |
| Date: | 31 March 2015 |
| Official Publication: | https://doi.org/10.1093/nar/gkv167 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
