![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of Accepted manuscript]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF (Accepted manuscript)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
13MB |
| Item Type: | Article |
|---|---|
| Title: | Cell divisions are not essential for the direct conversion of fibroblasts into neuronal cells |
| Creators Name: | Fishman, V.S., Shnayder, T.A., Orishchenko, K.E., Bader, M., Alenina, N. and Serov, O.L. |
| Abstract: | Direct lineage conversion is a promising approach for disease modeling and regenerative medicine. Cell divisions play a key role in reprogramming of somatic cells to pluripotency, however their role in direct lineage conversion is not clear. Here we used transdifferentiation of fibroblasts into neuronal cells by forced expression of defined transcription factors as a model system to study the role of cellular division in the direct conversion process. We have shown that conversion occurs in the presence of the cell cycle inhibitors aphidicolin or mimosine. Moreover, overexpression of the cell cycle activator cMyc negatively influences the process of direct conversion. Overall, our results suggest that cell divisions are not essential for the direct conversion of fibroblasts into neuronal cells. |
| Keywords: | Direct Conversion, Transdifferentiation, Reprogramming, Neuron, Fibroblast, Cell Division, Animals, Mice |
| Source: | Cell Cycle |
| ISSN: | 1538-4101 |
| Publisher: | Landes Bioscience |
| Volume: | 14 |
| Number: | 8 |
| Page Range: | 1188-1196 |
| Date: | 15 April 2015 |
| Official Publication: | https://doi.org/10.1080/15384101.2015.1012875 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
