Increased aortic intimal proliferation due to MasR deletion in vitro

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Item Type:	Article
Title:	Increased aortic intimal proliferation due to MasR deletion in vitro
Creators Name:	Alsaadon, H., Kruzliak, P., Smardencas, A., Hayes, A., Bader, M., Angus, P., Herath, C. and Zulli, A.
Abstract:	A growing body of evidence suggests that the vascular actions of Ang-(1-7) appear to involve increased production of nitric oxide (NO), an important vasodilator, through the activation of MasR, thus indicating the involvement of the MasR in preventing endothelial dysfunction. However, it is unknown whether the MasR could be involved in the progression of the next step in atherosclerosis, neo-intimal formation. To determine whether the deletion of the MasR is involved in the development of intimal thickening in an in vitro model. Mice [three background controls (C57Bl/6) and 3 MasR (-/-)] were killed and the aortas excised and cleaned of connective tissue and cut into 3 mm rings. Rings were placed in an organ culture medium for 5 weeks, embedded in paraffin, cut at 5 μm and stained with haematoxylin and eosin and Masson's trichrome. In addition, aortic reactivity was measured in organ baths. After 5 weeks of culture, the intima:media ratio increased in the aortas from MasR (-/-) mice compared to the control group by 4.5-fold (P < 0.01). However, no significant difference in nuclei area count (cell proliferation) between the MasR (-/-) mice and control group was observed (0.87 +/- 0.29% vs. 0.94 +/- 0.18%, respectively, P = ns). Functional studies showed only a minor vasoconstrictive and full vasodilative response. This study shows that the deletion of the MasR causes marked increase in the aortic intima:media ratio, which is not due to generalized cellular proliferation. These results provide a functional role for the MasR in atherogenesis.
Keywords:	Angiotensin (1-7) Mas Receptor, Atherosclerosis, Endothelial Function, Intimal Thickening, Animals, Mice
Source:	International Journal of Experimental Pathology
ISSN:	0959-9673
Publisher:	Wiley-Blackwell
Volume:	96
Number:	3
Page Range:	183-187
Date:	June 2015
Official Publication:	https://doi.org/10.1111/iep.12118
PubMed:	View item in PubMed

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