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| Item Type: | Article |
|---|---|
| Title: | Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium |
| Creators Name: | Lluch-Senar, M., Delgado, J., Chen, W.-H., Llorens-Rico, V., O'Reilly, F.J., Wodke, J.A.H., Unal, E.B., Yus, E., Martinez, S., Nichols, R.J., Ferrar, T., Vivancos, A., Schmeisky, A., Stuelke, J., van Noort, V., Gavin, A.-C., Bork, P. and Serrano, L. |
| Abstract: | Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%), followed by conventional ORFs (49%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs (ncRNAs) non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. We found that the minimal essential genome is comprised of 33% (269,410 bp) of the M. pneumoniae genome. Our data highlight an unexpected hidden layer of smORFs with essential functions, as well as non-coding regions, thus changing the focus when aiming to define the minimal essential genome. |
| Keywords: | Minimal Genome, Non-Coding RNAs, Small Proteins |
| Source: | Molecular Systems Biology |
| ISSN: | 1744-4292 |
| Publisher: | EMBO Press / Wiley |
| Volume: | 11 |
| Number: | 1 |
| Page Range: | 780 |
| Date: | 21 January 2015 |
| Official Publication: | https://doi.org/10.15252/msb.20145558 |
| PubMed: | View item in PubMed |
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