The subpopulation of microglia expressing functional muscarinic acetylcholine receptors expands in stroke and Alzheimer's disease

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Item Type:	Article
Title:	The subpopulation of microglia expressing functional muscarinic acetylcholine receptors expands in stroke and Alzheimer's disease
Creators Name:	Pannell, M., Meier, M.A., Szulzewsky, F., Matyash, V., Endres, M., Kronenberg, G., Prinz, V., Waiczies, S., Wolf, S.A. and Kettenmann, H.
Abstract:	Microglia undergo a process of activation in pathology which is controlled by many factors including neurotransmitters. We found that a subpopulation (11 %) of freshly isolated adult microglia respond to the muscarinic acetylcholine receptor agonist carbachol with a Ca(2+) increase and a subpopulation of similar size (16 %) was observed by FACS analysis using an antibody against the M3 receptor subtype. The carbachol-sensitive population increased in microglia/brain macrophages isolated from tissue of mouse models for stroke (60 %) and Alzheimer's disease (25 %), but not for glioma and multiple sclerosis. Microglia cultured from adult and neonatal brain contained a carbachol-sensitive subpopulation (8 and 9 %), which was increased by treatment with interferon-{gamma} to around 60 %. This increase was sensitive to blockers of protein synthesis and correlated with an upregulation of the M3 receptor subtype and with an increased expression of MHC-I and MHC-II. Carbachol was a chemoattractant for microglia and decreased their phagocytic activity.
Keywords:	Muscarinic Acetylcholine Receptors, Microglia, Brain Macrophages, Stroke, Alzheimer's Disease, Glioma, Multiple Sclerosis, Animals, Mice
Source:	Brain Structure & Function
ISSN:	1863-2653
Publisher:	Springer
Volume:	221
Number:	2
Page Range:	1157-1172
Date:	March 2016
Official Publication:	https://doi.org/10.1007/s00429-014-0962-y
PubMed:	View item in PubMed

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