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Promoter architecture of mouse olfactory receptor genes

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Item Type:Article
Title:Promoter architecture of mouse olfactory receptor genes
Creators Name:Plessy, C., Pascarella, G., Bertin, N., Akalin, A., Carrieri, C., Vassalli, A., Lazarevic, D., Severin, J., Vlachouli, C., Simone, R., Faulkner, G.J., Kawai, J., Daub, C.O., Zucchelli, S., Hayashizaki, Y., Mombaerts, P., Lenhard, B., Gustincich, S. and Carninci, P.
Abstract:Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR gene Olfr160 (M72) can drive OSN-specific expression in transgenic mice.
Keywords:3' Untranslated Regions, Base Sequence, Binding Sites, Consensus Sequence, DNA-Binding Proteins, Gene Expression Profiling, Gene Expression Regulation, Gene Order, Genetic Loci, Genetic Promoter Regions, Genetic Transcription, MEF2 Transcription Factors, Myogenic Regulatory Factors, Odorant Receptors, Olfactory Mucosa, Olfactory Receptor Neurons, TATA-Box Binding Protein, Transcription Factors, Transcription Initiation Site, Transgenic Mice, Animals, Mice
Source:Genome Research
Publisher:Cold Spring Harbor Laboratory Press
Page Range:486-497
Date:March 2012
Official Publication:https://doi.org/10.1101/gr.126201.111
PubMed:View item in PubMed

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