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Assessment of serum protein dynamics by native SILAC flooding (SILflood)

Item Type:Article
Title:Assessment of serum protein dynamics by native SILAC flooding (SILflood)
Creators Name:Nolte, H., Hölper, S., Selbach, M., Braun, T. and Krüger, M.
Abstract:Turnover of blood serum proteins is a vital function in mammals, but technical challenges have thus far prevented comprehensive measurements of serum protein half-lives. Here, we injected native serum from heavy stable isotope labeled (SIL) mice into non-labeled recipients to quantify turnover of more than 200 proteins using mass spectrometry with high reproducibility and accuracy. We found a median of 19.4 h and a total range of 6-70 h for calculated half-lives. Moreover, we observed for proteins with equal function similar half-lives. To demonstrate the value and effectiveness of SILflood we investigated the impaired serum clearance in {beta}2-microglobulin (B2M-/-) deficient mice. Notably, we found that serum albumin and IgG half-lives were clearly reduced in B2M-/- animals compared to control animals. Taken together, our results demonstrate that SILflood is a versatile tool to investigate serum half-lives under regular and pathological conditions in living animals.
Keywords:Blood Serum Protein Turnover, B2M Knock Out, SILAC Mouse, Quantitative Proteomics, Animals, Mice
Source:Analytical Chemistry
Publisher:American Chemical Society
Page Range:11033-11037
Date:18 November 2014
Official Publication:https://doi.org/10.1021/ac502883p
PubMed:View item in PubMed

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