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Blood flow and Bmp signaling control endocardial chamber morphogenesis

Item Type:Article
Title:Blood flow and Bmp signaling control endocardial chamber morphogenesis
Creators Name:Dietrich, A.C., Lombardo, V.A., Veerkamp, J., Priller, F. and Abdelilah-Seyfried, S.
Abstract:During heart development, the onset of heartbeat and blood flow coincides with a ballooning of the cardiac chambers. Here, we have used the zebrafish as a vertebrate model to characterize chamber ballooning morphogenesis of the endocardium, a specialized population of endothelial cells that line the interior of the heart. By combining functional manipulations, fate mapping studies, and high-resolution imaging, we show that endocardial growth occurs without an influx of external cells. Instead, endocardial cell proliferation is regulated, both by blood flow and by Bmp signaling, in a manner independent of vascular endothelial growth factor (VEGF) signaling. Similar to myocardial cells, endocardial cells obtain distinct chamber-specific and inner- versus outer-curvature-specific surface area sizes. We find that the hemodynamic-sensitive transcription factor Klf2a is involved in regulating endocardial cell morphology. These findings establish the endocardium as the flow-sensitive tissue in the heart with a key role in adapting chamber growth in response to the mechanical stimulus of blood flow.
Keywords:Bone Morphogenetic Proteins, Cell Movement, Cell Proliferation, Endocardium, Endothelial Cells, Hemodynamics, Kruppel-Like Transcription Factors, Morphogenesis, Signal Transduction, Vascular Endothelial Growth Factor A, Zebrafish Proteins, Animals, Zebrafish
Source:Developmental Cell
Publisher:Cell Press
Page Range:367-377
Date:25 August 2014
Additional Information:Erratum in: Dev Cell 32(3): 387.
Official Publication:https://doi.org/10.1016/j.devcel.2014.06.020
PubMed:View item in PubMed

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