![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of Article]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF (Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
898kB |
![[thumbnail of Supplemental Material]](https://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplemental Material)
4MB |
| Item Type: | Article |
|---|---|
| Title: | Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets |
| Creators Name: | Farazi, T.A., Leonhardt, C.S., Mukherjee, N., Mihailovic, A., Li, S., Max, K.E.A., Meyer, C., Yamaji, M., Cekan, P., Jacobs, N.C., Gerstberger, S., Bognanni, C., Larsson, E., Ohler, U. and Tuschl, T. |
| Abstract: | Recent studies implicated the RNA-binding protein with multiple splicing (RBPMS) family of proteins in oocyte, retinal ganglion cell, heart, and gastrointestinal smooth muscle development. These RNA-binding proteins contain a single RNA recognition motif (RRM), and their targets and molecular function have not yet been identified. We defined transcriptome-wide RNA targets using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in HEK293 cells, revealing exonic mature and intronic pre-mRNA binding sites, in agreement with the nuclear and cytoplasmic localization of the proteins. Computational and biochemical approaches defined the RNA recognition element (RRE) as a tandem CAC trinucleotide motif separated by a variable spacer region. Similar to other mRNA-binding proteins, RBPMS family of proteins relocalized to cytoplasmic stress granules under oxidative stress conditions suggestive of a support function for mRNA localization in large and/or multinucleated cells where it is preferentially expressed. |
| Keywords: | RNA-Binding Protein, PAR-CLIP, RNA Recognition Element, RBPMS, Animals, Mice |
| Source: | RNA |
| ISSN: | 1355-8382 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Volume: | 20 |
| Number: | 7 |
| Page Range: | 1090-1102 |
| Date: | July 2014 |
| Official Publication: | https://doi.org/10.1261/rna.045005.114 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
