Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4

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Item Type:	Article
Title:	Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4
Creators Name:	Bachmann, M.F., Schorle, H., Kuehn, R., Mueller, W., Hengartner, H., Zinkernagel, R.M. and Horak, I.
Abstract:	Antiviral immune responses of mice lacking interleukin-2 (IL-2) or IL-4 or both IL-2 and IL-4 (IL-2/4) were compared by using different viruses. Primary cytotoxic T-lymphocyte (CTL) responses against lymphocytic choriomeningitis virus (LCMV) were only moderately reduced in mice lacking IL-2 and were normal in mice lacking IL-4. Mice deficient in both interleukins exhibited variable and more strongly reduced but nevertheless in vivo protective LCMV-specific CTL responses. Similar results were obtained with vaccinia virus. Upon virus-specific restimulation in vitro, spleen cells from IL-2- and IL-2/4-deficient mice failed to generate CTL responses against virus-infected target cells, whereas the response of mice deficient in only IL-4 was comparable to that of control mice. The addition of IL-2 during in vitro restimulation completely restored the responses of both IL-2 and IL-2/4-deficient mice. T-helper-cell-independent immunoglobulin M and T-helper-cell-dependent immunoglobulin G antibody responses against vesicular stomatitis virus glycoprotein were within normal ranges for the various mutant mice. After LCMV infection, specific antibody responses against LCMV nucleoprotein were reduced four- to eightfold. These results show that mice lacking IL-2/4 have an overall tendency to exhibit more severely reduced CTL responses than IL-2- or IL-4-deficient mice. Nevertheless, and surprisingly, in vivo protective immune responses were mounted in the absence of IL-2/4, suggesting that besides a minor contribution from IL-4, other interleukins compensate in vivo for the lack of IL-2 in IL-2-deficient mice.
Keywords:	B-Lymphocytes, Cellular Immunity, Cytotoxic T-Lymphocytes, Genetic Crosses, Helper-Inducer T-Lymphocytes, Interleukin-2, Interleukin-4, Lymphocytic Choriomeningitis, Lymphocytic Choriomeningitis Virus, Vaccinia Virus, Viral Antibodies, Animals, Mice
Source:	Journal of Virology
ISSN:	0022-538X
Publisher:	American Society for Microbiology
Volume:	69
Number:	8
Page Range:	4842-4846
Date:	August 1995
Official Publication:	https://doi.org/10.1128/jvi.69.8.4842-4846.1995
PubMed:	View item in PubMed

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