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| Item Type: | Article |
|---|---|
| Title: | Sall4 isoforms act during proximal-distal and anterior-posterior axis formation in the mouse embryo |
| Creators Name: | Uez, N., Lickert, H., Kohlhase, J., de Angelis, M.H., Kühn, R., Wurst, W. and Floss, T. |
| Abstract: | Reciprocal signals from embryonic and extra-embryonic tissues pattern the embryo in proximal-distal (PD) and anterior-posterior (AP) fashion. Here we have analyzed three gene trap mutations of Sall4, of which one (Sall4-1a) led to a hypomorphic and recessive phenotype, demonstrating that Sall4-1a has yet undescribed extra-embryonic and embryonic functions in regulating PD and AP axis formation. In Sall4-1a mutants the self-maintaining autoregulatory interaction between Bmp4, Nodal and Wnt, which determines the PD axis was disrupted because of defects in the extra-embryonic visceral endoderm. More severely, two distinct Sall4 gene-trap mutants (Sall4-1a,b), resembling null mutants, failed to initiate Bmp4 expression in the extra-embryonic ectoderm and Nodal in the epiblast and were therefore unable to initiate PD axis formation. Tetraploid rescue underlined the extra-embryonic nature of the Sall4-1a phenotype and revealed a further embryonic function in Wnt/beta-catenin signaling to elongate the AP axis during gastrulation. This observation was supported through genetic interaction with beta-catenin mutants, since compound heterozygous mutants recapitulated the defects of Wnt3a mutants in posterior development. |
| Keywords: | Sall4, Spalt, Gene Trap, Axis Formation, Axis Elongation, Animals, Mice |
| Source: | Genesis |
| ISSN: | 1526-954X |
| Publisher: | Wiley |
| Volume: | 46 |
| Number: | 9 |
| Page Range: | 463-477 |
| Date: | September 2008 |
| Official Publication: | https://doi.org/10.1002/dvg.20421 |
| PubMed: | View item in PubMed |
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