![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | Protein composition of the human heart: the construction of a myocardial two-dimensional electrophoresis database |
| Creators Name: | Jungblut, P., Otto, A., Zeindl-Eberhart, E., Pleissner, K.P., Knecht, M., Regitz-Zagrosek, V., Fleck, E. and Wittmann-Liebold, B. |
| Abstract: | Molecular changes occurring in myocardial diseases are not well understood. Proteins, as regulatory molecules, should play an important role in the etiology of these diseases. The method of two-dimensional electrophoresis (2-DE) allows the analysis of some thousand proteins with one experiment. An important prerequisite for this kind of investigation is the possibility of identifying the proteins separated by 2-DE. We resolve 3239 proteins of the human myocardium and tried to identify 33 proteins by amino acid analysis and microsequencing. Twenty proteins were identified by search for the protein-chemical data obtained in the Martinsried Institute Protein Sequence Database. Comparisons of 2-DE patterns of different size, which were obtained in different laboratories, were performed with the result that proteins identified on a 2-DE map of one laboratory can be assigned to spots of 2-DE maps produced by another laboratory. Our results show the usefulness of a myocardial 2-DE database; they can be used in different laboratories and make it possible to generate a collection of important human myocardial proteins in a 2-DE database for comparative studies worldwide. |
| Keywords: | Amino Acid Sequence, Amino Acids, Autoanalysis, Dilated Cardiomyopathy, Factual Databases, Gel, Two-Dimensional Electrophoresis, Isoelectric Focusing, Laboratories, Molecular Sequence Data, Myocardium, Proteins |
| Source: | Electrophoresis |
| ISSN: | 0173-0835 |
| Publisher: | Wiley |
| Volume: | 15 |
| Number: | 5 |
| Page Range: | 685-707 |
| Date: | 1 May 1994 |
| Official Publication: | https://doi.org/10.1002/elps.1150150197 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
