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| Item Type: | Article |
|---|---|
| Title: | Distinct polyadenylation landscapes of diverse human tissues revealed by a modified PA-seq strategy |
| Creators Name: | Ni, T., Yang, Y., Hafez, D., Yang, W., Kiesewetter, K., Wakabayashi, Y., Ohler, U., Peng, W. and Zhu, J. |
| Abstract: | Background: Polyadenylation is a key regulatory step in eukaryotic gene expression and one of the major contributors of transcriptome diversity. Aberrant polyadenylation often associates with expression defects and leads to human diseases. Results: To better understand global polyadenylation regulation, we have developed a polyadenylation sequencing (PA-seq) approach. By profiling polyadenylation events in 13 human tissues, we found that alternative cleavage and polyadenylation (APA) is prevalent in both protein-coding and noncoding genes. In addition, APA usage, similar to gene expression profiling, exhibits tissue-specific signatures and is sufficient for determining tissue origin. A 3? untranslated region shortening index (USI) was further developed for genes with tandem APA sites. Strikingly, the results showed that different tissues exhibit distinct patterns of shortening and/or lengthening of 3? untranslated regions, suggesting the intimate involvement of APA in establishing tissue or cell identity. Conclusions: This study provides a comprehensive resource to uncover regulated polyadenylation events in human tissues and to characterize the underlying regulatory mechanism. |
| Keywords: | 3' Untranslated Regions, Chromosome Mapping, Cluster Analysis, Gene Library, Organ Specificity, Polyadenylation, RNA Sequence Analysis |
| Source: | BMC Genomics |
| ISSN: | 1471-2164 |
| Publisher: | BioMed Central |
| Volume: | 14 |
| Number: | 1 |
| Page Range: | 615 |
| Date: | 11 September 2013 |
| Official Publication: | https://doi.org/10.1186/1471-2164-14-615 |
| PubMed: | View item in PubMed |
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