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| Item Type: | Article |
|---|---|
| Title: | Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment |
| Creators Name: | Alder, O., Lavial, F., Helness, A., Brookes, E., Pinho, S., Chandrashekran, A., Arnaud, P., Pombo, A., O'Neill, L. and Azuara, V. |
| Abstract: | Pluripotent cells develop within the inner cell mass of blastocysts, a mosaic of cells surrounded by an extra-embryonic layer, the trophectoderm. We show that a set of somatic lineage regulators (including Hox, Gata and Sox factors) that carry bivalent chromatin enriched in H3K27me3 and H3K4me2 are selectively targeted by Suv39h1-mediated H3K9me3 and de novo DNA methylation in extra-embryonic versus embryonic (pluripotent) lineages, as assessed both in blastocyst-derived stem cells and in vivo. This stably repressed state is linked with a loss of gene priming for transcription through the exclusion of PRC1 (Ring1B) and RNA polymerase II complexes at bivalent, lineage-inappropriate genes upon trophoblast lineage commitment. Collectively, our results suggest a mutually exclusive role for Ring1B and Suv39h1 in regulating distinct chromatin states at key developmental genes and propose a novel mechanism by which lineage specification can be reinforced during early development. |
| Keywords: | Bivalent Chromatin, Early Development, Histone Methylation, Mouse, Silencing, Stem Cells, Animals, Mice |
| Source: | Development |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Volume: | 137 |
| Number: | 15 |
| Page Range: | 2483-2492 |
| Date: | 1 August 2010 |
| Official Publication: | https://doi.org/10.1242/dev.048363 |
| PubMed: | View item in PubMed |
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