Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment

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Item Type:	Article
Title:	Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment
Creators Name:	Alder, O., Lavial, F., Helness, A., Brookes, E., Pinho, S., Chandrashekran, A., Arnaud, P., Pombo, A., O'Neill, L. and Azuara, V.
Abstract:	Pluripotent cells develop within the inner cell mass of blastocysts, a mosaic of cells surrounded by an extra-embryonic layer, the trophectoderm. We show that a set of somatic lineage regulators (including Hox, Gata and Sox factors) that carry bivalent chromatin enriched in H3K27me3 and H3K4me2 are selectively targeted by Suv39h1-mediated H3K9me3 and de novo DNA methylation in extra-embryonic versus embryonic (pluripotent) lineages, as assessed both in blastocyst-derived stem cells and in vivo. This stably repressed state is linked with a loss of gene priming for transcription through the exclusion of PRC1 (Ring1B) and RNA polymerase II complexes at bivalent, lineage-inappropriate genes upon trophoblast lineage commitment. Collectively, our results suggest a mutually exclusive role for Ring1B and Suv39h1 in regulating distinct chromatin states at key developmental genes and propose a novel mechanism by which lineage specification can be reinforced during early development.
Keywords:	Bivalent Chromatin, Early Development, Histone Methylation, Mouse, Silencing, Stem Cells, Animals, Mice
Source:	Development
ISSN:	0950-1991
Publisher:	Company of Biologists
Volume:	137
Number:	15
Page Range:	2483-2492
Date:	1 August 2010
Official Publication:	https://doi.org/10.1242/dev.048363
PubMed:	View item in PubMed

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