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| Item Type: | Article |
|---|---|
| Title: | Loss of epidermal Evi/Wls results in a phenotype resembling psoriasiform dermatitis |
| Creators Name: | Augustin, I., Gross, J., Baumann, D., Korn, C., Kerr, G., Grigoryan, T., Mauch, C., Birchmeier, W. and Boutros, M. |
| Abstract: | Cells of the epidermis renew constantly from germinal layer stem cells. Although epithelial cell differentiation has been studied in great detail and the role of Wnt signaling in this process is well described, the contribution of epidermal Wnt secretion in epithelial cell homeostasis remains poorly understood. To analyze the role of Wnt proteins in this process, we created a conditional knockout allele of the Wnt cargo receptor Evi/Gpr177/Wntless and studied mice that lacked Evi expression in the epidermis. We found that K14-Cre, Evi-LOF mice lost their hair during the first hair cycle, showing a reddish skin with impaired skin barrier function. Expression profiling of mutant and wild-type skin revealed up-regulation of inflammation-associated genes. Furthermore, we found that Evi expression in psoriatic skin biopsies is down-regulated, suggesting that Evi-deficient mice developed skin lesions that resemble human psoriasis. Immune cell infiltration was detected in Evi-LOF skin. Interestingly, an age-dependent depletion of dendritic epidermal T cells (DETCs) and an infiltration of gammadelta(low) T cells in Evi mutant epidermis was observed. Collectively, the described inflammatory skin phenotype in Evi-deficient mice revealed an essential role of Wnt secretion in maintaining normal skin homeostasis by enabling a balanced epidermal-dermal cross talk, which affects immune cell recruitment and DETC survival. |
| Keywords: | CD3 Antigens, Cell Proliferation, Chronic Disease, Dendritic Cells, Dermatitis, Epidermis, G-Protein-Coupled Receptors, gamma-delta T-Cell Antigen Receptors, Gene Deletion, Inflammation, Intracellular Signaling Peptides and Proteins, Keratinocytes, Keratins, Lymphocyte Activation, Neutrophil Infiltration, Phenotype, Psoriasis, STAT3 Transcription Factor, T-Lymphocytes, Wnt Proteins, Animals, Mice |
| Source: | Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Volume: | 210 |
| Number: | 9 |
| Page Range: | 1761-1777 |
| Date: | 26 August 2013 |
| Official Publication: | https://doi.org/10.1084/jem.20121871 |
| PubMed: | View item in PubMed |
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