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| Item Type: | Article |
|---|---|
| Title: | A genetic strategy for the analysis of individual axon morphologies in cGMP signalling mutant mice |
| Creators Name: | Schmidt, H., Ter-Avetisyan, G. and Rathjen, F.G. |
| Abstract: | One of the many physiological functions of cyclic guanosine 3',5' monophosphate (cGMP) signalling is the regulation of a specific mode of axonal branching. The bifurcation of axons from dorsal root ganglion (DRG) neurons at the dorsal root entry zone of the embryonic spinal cord is triggered by a cGMP -signalling pathway comprising the ligand C-type natriuretic peptide (CNP), the cGMP-producing natriuretic peptide receptor 2 (Npr2), and the cGMP-dependent protein kinase Ialpha (cGKIalpha). Absence of any of these components causes a loss of bifurcation and sensory axons instead only turn in either a rostral or a caudal direction. In this chapter we describe a genetic strategy to study the impact of cGMP signalling on the arborization of individual DRG neurons in mice. Expression of an alkaline phosphatase (AP) reporter is selectively induced in Npr2-positive DRG neurons by tamoxifen-dependent activation of a Cre -recombinase under the control of the Npr2 promoter. This approach might also be employed for the analysis of axonal branching in neuronal subsets expressing Npr2 elsewhere in the nervous system. |
| Keywords: | Axonal Branching, DRG, Sparse Neuronal Labelling, CNP, Npr2, cGKI, cGMP, Tamoxifen, Cre Recombinase, Animals, Mice |
| Source: | Methods in Molecular Biology |
| Series Name: | Methods in Molecular Biology |
| Title of Book: | Guanylate cyclase and cyclic GMP : methods and protocols |
| ISSN: | 1064-3745 |
| ISBN: | 978-1-62703-459-3 |
| Publisher: | Springer / Humana Press |
| Volume: | 1020 |
| Page Range: | 193-204 |
| Number of Pages: | 249 |
| Date: | 2013 |
| Official Publication: | https://doi.org/10.1007/978-1-62703-459-3_12 |
| PubMed: | View item in PubMed |
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