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The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression

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Item Type:Article
Title:The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression
Creators Name:Chekulaeva, M., Parker, R. and Filipowicz, W.
Abstract:The control of messenger RNA (mRNA) function by micro RNAs (miRNAs) in animal cells requires the GW182 protein. GW182 is recruited to the miRNA repression complex via interaction with Argonaute protein, and functions downstream to repress protein synthesis. Interaction with Argonaute is mediated by GW/WG repeats, which are conserved in many Argonaute-binding proteins involved in RNA interference and miRNA silencing, from fission yeast to mammals. GW182 contains at least three effector domains that function to repress target mRNA. Here, we analyze the functions of the N-terminal GW182 domain in repression and Argonaute1 binding, using tethering and immunoprecipitation assays in Drosophila cultured cells. We demonstrate that its function in repression requires intact GW/WG repeats, but does not involve interaction with the Argonaute1 protein, and is independent of the mRNA polyadenylation status. These results demonstrate a novel role for the GW/WG repeats as effector motifs in miRNA-mediated repression.
Keywords:Amino Acid Sequence, Amino Acid Repetitive Sequences, Argonaute Proteins, Cultured Cells, Drosophila Proteins, Eukaryotic Initiation Factors, Messenger RNA, MicroRNAs, Molecular Sequence Data, RNA-Binding Proteins, RNA Interference, Tertiary Protein Structure, Animals, Drosophila
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press
Volume:38
Number:19
Page Range:6673-6683
Date:October 2010
Official Publication:https://doi.org/10.1093/nar/gkq501
PubMed:View item in PubMed

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