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| Item Type: | Article |
|---|---|
| Title: | Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells |
| Creators Name: | Marguerat, S., Schmidt, A., Codlin, S., Chen, W., Aebersold, R. and Baehler, J. |
| Abstract: | Data on absolute molecule numbers will empower the modeling, understanding, and comparison of cellular functions and biological systems. We quantified transcriptomes and proteomes in fission yeast during cellular proliferation and quiescence. This rich resource provides the first comprehensive reference for all RNA and most protein concentrations in a eukaryote under two key physiological conditions. The integrated data set supports quantitative biology and affords unique insights into cell regulation. Although mRNAs are typically expressed in a narrow range above 1 copy/cell, most long, noncoding RNAs, except for a distinct subset, are tightly repressed below 1 copy/cell. Cell-cycle-regulated transcription tunes mRNA numbers to phase-specific requirements but can also bring about more switch-like expression. Proteins greatly exceed mRNAs in abundance and dynamic range, and concentrations are regulated to functional demands. Upon transition to quiescence, the proteome changes substantially, but, in stark contrast to mRNAs, proteins do not uniformly decrease but scale with cell volume. |
| Keywords: | Cell Cycle, Fungal RNA, Long Untranslated RNA, Mass Spectrometry, Messenger RNA, Proteome, RNA Sequence Analysis, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Transcriptome |
| Source: | Cell |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Volume: | 151 |
| Number: | 3 |
| Page Range: | 671-683 |
| Date: | 26 October 2012 |
| Official Publication: | https://doi.org/10.1016/j.cell.2012.09.019 |
| PubMed: | View item in PubMed |
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