The protein expression landscape of the Arabidopsis root

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Item Type:	Article
Title:	The protein expression landscape of the Arabidopsis root
Creators Name:	Petricka, J.J., Schauer, M.A., Megraw, M., Breakfield, N.W., Thompson, J.W., Georgiev, S., Soderblom, E.J., Ohler, U., Moseley, M.A., Grossniklaus, U. and Benfey, P.N.
Abstract:	Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of nearly 2,000 proteins. Grouping proteins into functional categories revealed unique cellular functions and identified cell type-specific biomarkers. Cellular colocalization provided support for numerous protein-protein interactions. With a binary comparison, we found that RNA and protein expression profiles are weakly correlated. We then performed peak integration at cell type-specific resolution and found an improved correlation with transcriptome data using continuous values. We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry) proteomic experiments on mutants with ectopic and no root hairs, providing complementary proteomic data. Finally, among our root hair-specific proteins we identified two unique regulators of root hair development.
Keywords:	Cell-Type Expression, FACS, Plant Proteome, RNA-Protein Correlation, Root Hair Mutant
Source:	Proceedings of the National Academy of Sciences of the United States of America
ISSN:	0027-8424
Publisher:	National Academy of Sciences
Volume:	109
Number:	18
Page Range:	6811-6818
Date:	1 May 2012
Official Publication:	https://doi.org/10.1073/pnas.1202546109
PubMed:	View item in PubMed

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