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| Item Type: | Article |
|---|---|
| Title: | The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts |
| Creators Name: | Baltz, A.G., Munschauer, M., Schwanhaeusser, B., Vasile, A., Murakawa, Y., Schueler, M., Youngs, N., Penfold-Brown, D., Drew, K., Milek, M., Wyler, E., Bonneau, R., Selbach, M., Dieterich, C. and Landthaler, M. |
| Abstract: | Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation, and translation. We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteomics and to display the protein occupancy on mRNA transcripts by next-generation sequencing. Application to a human embryonic kidney cell line identified close to 800 proteins. To our knowledge, nearly one-third were not previously annotated as RNA binding, and about 15% were not predictable by computational methods to interact with RNA. Protein occupancy profiling provides a transcriptome-wide catalog of potential cis-regulatory regions on mammalian mRNAs and showed that large stretches in 3' UTRs can be contacted by the mRNA-bound proteome, with numerous putative binding sites in regions harboring disease-associated nucleotide polymorphisms. Our observations indicate the presence of a large number of mRNA binders with diverse molecular functions participating in combinatorial posttranscriptional gene-expression networks. |
| Keywords: | Binding Sites, Cell Line, Mass Spectrometry, Messenger RNA, Proteomics, RNA-Binding Proteins, RNA Sequence Analysis |
| Source: | Molecular Cell |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Volume: | 46 |
| Number: | 5 |
| Page Range: | 674-690 |
| Date: | 8 June 2012 |
| Official Publication: | https://doi.org/10.1016/j.molcel.2012.05.021 |
| PubMed: | View item in PubMed |
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