In vivo quantitative proteome profiling: planning and evaluation of SILAC experiments

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Item Type:	Article
Title:	In vivo quantitative proteome profiling: planning and evaluation of SILAC experiments
Creators Name:	Kirchner, M. and Selbach, M.
Abstract:	Mass spectrometry-based quantitative proteomics can identify and quantify thousands of proteins in complex biological samples. Improved instrumentation, quantification strategies and data analysis tools now enable protein analysis on a genome-wide scale. Particularly, quantification based on stable isotope labeling with amino acids (SILAC) has emerged as a robust, reliable and simple method for accurate large-scale protein quantification. The spectrum of applications ranges from bacteria and eukaryotic cell culture systems to multicellular organisms. Here, we provide a step-by-step protocol on how to plan and perform large-scale quantitative proteome analysis using SILAC, from sample preparation to final data analysis.
Keywords:	In Vivo SILAC, Animal Model, MaxQuant, Large-Scale Proteomics, Quantitative Mass Spectrometry, Animals
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Quantative methods in proteomics
ISSN:	1064-3745
ISBN:	978-1-61779-884-9
Publisher:	Springer / Humana Press
Volume:	893
Page Range:	175-199
Number of Pages:	539
Date:	7 May 2012
Official Publication:	https://doi.org/10.1007/978-1-61779-885-6_13
PubMed:	View item in PubMed

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