Combined RNAi and localization for functionally dissecting long noncoding RNAs

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Item Type:	Article
Title:	Combined RNAi and localization for functionally dissecting long noncoding RNAs
Creators Name:	Chakraborty, D., Kappei, D., Theis, M., Nitzsche, A., Ding, L., Paszkowski-Rogacz, M., Surendranath, V., Berger, N., Schulz, H., Saar, K., Hubner, N. and Buchholz, F.
Abstract:	Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.
Keywords:	Embryonic Stem Cells, Fluorescence In Situ Hybridization, Gene Knockdown Techniques, RNA Interference, Untranslated RNA, Animals, Mice
Source:	Nature Methods
ISSN:	1548-7091
Publisher:	Nature Publishing Group
Volume:	9
Number:	4
Page Range:	360-362
Date:	12 February 2012
Official Publication:	https://doi.org/10.1038/nmeth.1894
PubMed:	View item in PubMed

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