Neuron-type specific regulation of a 3'UTR through redundant and combinatorially acting cis-regulatory elements

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Item Type:	Article
Title:	Neuron-type specific regulation of a 3'UTR through redundant and combinatorially acting cis-regulatory elements
Creators Name:	Didiano, D., Cochella, L., Tursun, B. and Hobert, O.
Abstract:	3' Untranslated region (UTR)-dependent post-transcriptional regulation has emerged as a critical mechanism of controlling gene expression in various physiological contexts, including cellular differentiation events. Here, we examine the regulation of the 3'UTR of the die-1 transcription factor in a single neuron of the nematode C. elegans. This 3'UTR shows the intriguing feature of being differentially regulated across the animal's left/right axis. In the left gustatory neuron, ASEL, in which DIE-1 protein is normally expressed in adult animals, the 3'UTR confers no regulatory information, while in the right gustatory neuron, ASER, where DIE-1 is normally not expressed, this 3'UTR confers negative regulatory information. Here, we systematically analyze the cis-regulatory architecture of the die-1 3'UTR using a transgenic, in vivo assay system. Through extensive mutagenesis and sequence insertions into heterologous 3'UTR contexts, we describe three 25-base-pair (bp) sequence elements that are both required and sufficient to mediate the ASER-specific down-regulation of the die-1 3'UTR. These three 25-bp sequence elements operate in both a redundant and combinatorial manner. Moreover, there are not only redundant elements within the die-1 3'UTR regulating its left/right asymmetric activity but asymmetric 3'UTR regulation is itself redundant with other regulatory mechanisms to achieve asymmetric DIE-1 protein expression and function in ASEL versus ASER. The features of 3'UTR regulation we describe here may apply to some of the vast number of genes in animal genomes whose expression is predicted to be regulated through their 3'UTR.
Keywords:	C. elegans, 3'UTR, miRNA, Neuronal Cell Fate, Cis-Regulatory Elements, Animals, Caenorhabditis elegans
Source:	RNA
ISSN:	1355-8382
Publisher:	Cold Spring Harbor Laboratory Press
Volume:	16
Number:	2
Page Range:	349-363
Date:	February 2010
Official Publication:	https://doi.org/10.1261/rna.1931510
PubMed:	View item in PubMed

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