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| Item Type: | Review |
|---|---|
| Title: | Transcriptome-wide analysis of protein-RNA interactions using high-throughput sequencing |
| Creators Name: | Milek, M., Wyler, E. and Landthaler, M. |
| Abstract: | Protein-RNA interactions are emerging as an important functional element in the regulation of gene expression. Cross-linking of proteins to RNA by UV irradiation followed by immunoprecipitation (CLIP) has provided a crucial tool for research in this field. Initially, the bottleneck of the method was the relatively low number of identified RNA binding sites. It was only the arrival of next-generation sequencing that allowed a comprehensive and unbiased description of the cross-linked protein-RNA fragments. Here, we summarize recent progress in the study of protein-RNA interactions, as well as some of the important findings obtained using different CLIP approaches in cultured cells and organisms. These efforts allowed the identification of functional RNA-binding sites for a wide range of RNA-interacting proteins. Experimental and bioinformatic progress will further advance this dynamic area of research. The combination of high-resolution protein-RNA interaction maps with transcriptome-wide data describing the stability, modifications and structures of RNAs, in addition to protein expression profiling, will provide deeper insight into post-transcriptional and translational regulatory events and mechanisms. |
| Keywords: | Post-Transcriptional Regulation, Protein-RNA Interactions, Cross-Linking and Immunoprecipitation, CLIP, Next-Generation Sequencing, Animals |
| Source: | Seminars in Cell & Developmental Biology |
| ISSN: | 1084-9521 |
| Publisher: | Elsevier / Academic Press |
| Volume: | 23 |
| Number: | 2 |
| Page Range: | 206-212 |
| Date: | April 2012 |
| Official Publication: | https://doi.org/10.1016/j.semcdb.2011.12.001 |
| PubMed: | View item in PubMed |
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