Expression of MUC2-mucin in colorectal adenomas and carcinomas of different histological types

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Item Type:	Article
Title:	Expression of MUC2-mucin in colorectal adenomas and carcinomas of different histological types
Creators Name:	Blanke, M., Klussmann, E., Krueger-Krasagakes, S., Schmitt-Graeff, A., Stolte, M., Bornhoeft, G., Stein, H., Xing, P.X., McKenzie, I.F., Verstijnen, C.P., Riecken, E.O. and Hanski, C.
Abstract:	The expression of mucin MUC2 was investigated in normal colonic tissue, in colonic adenomas and in carcinomas of the mucinous and non-mucinous type. The latter were subdivided into carcinomas originating from the adenoma-carcinoma sequence (ACS) and de novo (DN) carcinomas. The expression was assayed by immunohistochemistry with the monoclonal anti-MUC2 antibody CCP58 and by mRNA semiquantitation. MUC2 protein epitope CCP58 was strongly expressed in 21% of normal colonic tissues, in 40% of villous and in 48% of tubular adenomas. Mucinous carcinomas exhibited strong expression in 72%, ACS carcinomas in 21% and DN adenocarcinomas in none of the tumors investigated. Compared with the adjacent non-malignant tissue (transitional mucosa), CCP58 epitope expression in the tumor was higher in 74% of mucinous carcinomas, but equal or lower in 69% of ACS carcinomas and in 100% of de novo carcinomas. The alterations of MUC2 expression detected by immunohistochemistry in adenocarcinomas were confirmed on mRNA level. These data indicate that the MUC2 expression pattern is different in the 3 carcinoma types investigated. MUC2 over-expression occurs in the adenomatous tissue. It is always maintained in mucinous carcinomas, but frequently decreased in non-mucinous ACS carcinomas. DN carcinomas are most frequently associated with decreased expression of MUC2.
Keywords:	Mucinous Adenocarcinoma, Villous Adenoma, Adenomatous Polyps, Base Sequence, Colorectal Neoplasms, DNA Primers, Differential Diagnosis, Gene Expression, Immunoenzyme Techniques, Molecular Sequence Data, Mucin-2, Mucins, Polymerase Chain Reaction, Precancerous Conditions, Messenger RNA, Biological Tumor Markers
Source:	International Journal of Cancer
ISSN:	0020-7136
Publisher:	Wiley-Blackwell
Volume:	59
Number:	3
Page Range:	301-306
Date:	1 November 1994
Official Publication:	https://doi.org/10.1002/ijc.2910590302
PubMed:	View item in PubMed

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