Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue

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Item Type:	Article
Title:	Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue
Creators Name:	Hanski, C., Klussmann, E., Wang, J., Boehm, C., Ogorek, D., Hanski, M.L., Krueger-Krasagakes, S., Eberle, J., Schmitt-Graeff, A. and Riecken, E.O.
Abstract:	The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue.
Keywords:	Colon Carcinoma Associated Mucins, Sialyl-Le(x), Fucosyltransferases, AM-3
Source:	Glycoconjugate Journal
ISSN:	0282-0080
Publisher:	Springer
Volume:	13
Number:	5
Page Range:	727-733
Date:	October 1996
Official Publication:	https://doi.org/10.1007/BF00702336
PubMed:	View item in PubMed

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