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| Item Type: | Article |
|---|---|
| Title: | Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue |
| Creators Name: | Hanski, C., Klussmann, E., Wang, J., Boehm, C., Ogorek, D., Hanski, M.L., Krueger-Krasagakes, S., Eberle, J., Schmitt-Graeff, A. and Riecken, E.O. |
| Abstract: | The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue. |
| Keywords: | Colon Carcinoma Associated Mucins, Sialyl-Le(x), Fucosyltransferases, AM-3 |
| Source: | Glycoconjugate Journal |
| ISSN: | 0282-0080 |
| Publisher: | Springer |
| Volume: | 13 |
| Number: | 5 |
| Page Range: | 727-733 |
| Date: | October 1996 |
| Official Publication: | https://doi.org/10.1007/BF00702336 |
| PubMed: | View item in PubMed |
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