Molecular imaging of breast cancer in a transgene mouse model [Molekulare Bildgebung des Mammakarzinoms in einem transgenen Mausmodell]

Tools

Item Type:	Book Section
Title:	Molecular imaging of breast cancer in a transgene mouse model [Molekulare Bildgebung des Mammakarzinoms in einem transgenen Mausmodell]
Creators Name:	Jakob, J., Perlitz, C., Ebert, B., Klamm, U., Kemmner, W., Haensch, W., Macdonald, R., Licha, K., Schirner, M., Schlag, P.M. and Moesta, K.T.
Abstract:	Background: Purpose of the study was to detect early breast cancer and its precursors by in vivo optical molecular imaging in an animal model. Material and methods: Females of the transgene mouse strain WAP-TNP8 develop non-invasive and consequently invasive tumours of the mammary glands due to specific expression of the viral SV40 large tumour antigen induced by lactation. The molecular target for imaging was extradomain-b fibronectin (EDB-FN), which is associated with tumour angiogenesis. The optical probe was designed as a compound of an anti-EDB-FN antibody fragment and a near-infrared fluorescent dye. 30h after intravenous injection of the contrast agent, optical imaging was performed using a pulsed Laser system for excitation and an intensified CCD-camera to record fluorescence images. After optical molecular imaging all animals were sacrified and the tumours were examined histologically. Results: Initiated transgene female animals developed palpable masses of the mammary gland within 6 months (median 4 months). Imaging was performed in 5 animals with a total of 9 tumours (diameter 2–7mm, median 4mm). Applying optical molecular imaging 8 of 9 tumours were detected. The urogenital tract was contrasted unspecifically. Histological examination proved invasive epithelial tumours of the mammary gland in all cases. Conclusion: Breast cancer can be detected in vivo by near-infrared fluorescence molecular imaging targeting neoangiogenesis in a transgene mouse-model.
Source:	Chirurgisches Forum 2005
Series Name:	Deutsche Gesellschaft fuer Chirurgie
Title of Book:	Chirurgisches Forum 2005 : fuer experimentelle und klinische Forschung 122. Kongress der Deutschen Gesellschaft fuer Chirurgie Berlin, 05.04.-08.04.2005
Publisher:	Springer
Volume:	34
Page Range:	83-85
Date:	2005
Official Publication:	https://doi.org/10.1007/b137791

Repository Staff Only: item control page