Bacterial inclusion bodies of Alzheimer's disease beta-amyloid peptides can be employed to study native-like aggregation intermediate states

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Item Type:	Article
Title:	Bacterial inclusion bodies of Alzheimer's disease beta-amyloid peptides can be employed to study native-like aggregation intermediate states
Creators Name:	Dasari, M., Espargaro, A., Sabate, R., Lopez Del Amo, J.M., Fink, U., Grelle, G., Bieschke, J., Ventura, S. and Reif, B.
Abstract:	The structures of oligomeric intermediate states in the aggregation process of Alzheimer's disease beta-amyloid peptides have been the subject of debate for many years. Bacterial inclusion bodies contain large amounts of small heat shock proteins (sHSPs), which are highly homologous to those found in the plaques of the brains of Alzheimer's disease patients. sHSPs break down amyloid fibril structure in vitro and induce oligomeric assemblies. Prokaryotic protein overexpression thus mimics the conditions encountered in the cell under stress and allows the structures of Abeta aggregation intermediate states to be investigated under native-like conditions, which is not otherwise technically possible. We show that IB40/IB42 fulfil all the requirements to be classified as amyloids: they seed fibril growth, are Congo red positive and show characteristic beta-sheet-rich CD spectra. However, IB40 and IB42 are much less stable than fibrils formed in vitro and contain significant amounts of non-beta-sheet regions, as seen from FTIR studies. Quantitative analyses of solution-state NMR H/D exchange rates show that the hydrophobic cores involving residues V18-F19-F20 adopt beta-sheet conformations, whereas the C termini adopt alpha-helical coiled-coil structures. In the past, an alpha-helical intermediate-state structure has been postulated, but could not be verified experimentally. In agreement with the current literature, in which Abeta oligomers are described as the most toxic state of the peptides, we find that IB42 contains SDS-resistant oligomers that are more neurotoxic than Abeta42 fibrils. E. coli inclusion bodies formed by the Alzheimer's disease beta-amyloid peptides Abeta40 and Abeta42 thus behave structurally like amyloid aggregation intermediate states and open the possibility of studying amyloids in a native-like, cellular environment.
Keywords:	Amyloid beta-Peptides, Hydrogen/Deuterium Exchange, Inclusion Bodies, NMR Spectroscopy, Protein Structures
Source:	ChemBioChem
ISSN:	1439-4227
Publisher:	Wiley-VCH
Volume:	12
Number:	3
Page Range:	407-423
Date:	11 February 2011
Official Publication:	https://doi.org/10.1002/cbic.201000602
PubMed:	View item in PubMed

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