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No association of converting enzyme insertion/deletion polymorphism with immunoglobulin a glomerulonephritis

Item Type:Article
Title:No association of converting enzyme insertion/deletion polymorphism with immunoglobulin a glomerulonephritis
Creators Name:Schmidt, S., Stier, E., Hartung, R., Stein, G., Bahnisch, J., Woodroffe, A.J., Clarkson, A.R., Ponticelli, C., Campise, M., Mayer, G., Ganten, D. and Ritz, E.
Abstract:It has been recently reported that in type 1 diabetes the insertion/deletion (I/D) polymorphism of the angiotensin I-converting enzyme gene is associated with the presence of diabetic nephropathy. Tissue angiotensin I-converting enzyme is determined by I/D polymorphism, and it has been speculated that in diabetes differences of local angiotensin II availability determine the risk of renal disease. Since angiotensin II is thought to play an important role in the evolution of renal disease in general, we tested whether genotype distribution of the I/D polymorphism is also different in patients with immunoglobulin A-glomerulonephritis (IgA-GN). Furthermore we compared IgA-GN patients with (1) stable renal function or (2) terminal renal failure to investigate a potential role of the I/D polymorphism in the renal prognosis. We examined 122 patients with biopsy-confirmed IgA-GN who had stable renal function and 82 dialysis-dependent or transplanted patients with biopsy-confirmed IgA-GN. Furthermore, in 134 healthy individuals used as controls we analyzed the DNA for normal distribution of genotypes and allele frequencies. The polymorphic region was amplified using polymerase chain reaction with specific primers. Alleles were detected on 2% agarose gels. Genotype distributions and allele frequencies were not significantly different between controls and patients with IgA-GN and stable renal function. Furthermore, no significant difference in genotype distribution was detected between patients with IgA-GN and stable renal function compared with patients with IgA-GN and end-stage renal failure, although a trend for a higher frequency of DD-homozygotes was noted in the latter group (P = 0.07).
Keywords:Alleles, IGA Glomerulonephritis, Kidney, Peptidyl-Dipeptidase A, Polymerase Chain Reaction, Genetic Polymorphism
Source:American Journal of Kidney Diseases
Page Range:727-731
Date:1 November 1995
Official Publication:https://doi.org/10.1016/0272-6386(95)90435-2
PubMed:View item in PubMed

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