![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of 11161oa.pdf]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
927kB |
| Item Type: | Article |
|---|---|
| Title: | Intergenic and repeat transcription in human, chimpanzee and macaque brains measured by RNA-Seq |
| Creators Name: | Xu, A.G., He, L., Li, Z., Xu, Y., Li, M., Fu, X., Yan, Z., Yuan, Y., Menzel, C., Li, N., Somel, M., Hu, H., Chen, W., Paeaebo, S. and Khaitovich, P. |
| Abstract: | Transcription is the first step connecting genetic information with an organism's phenotype. While expression of annotated genes in the human brain has been characterized extensively, our knowledge about the scope and the conservation of transcripts located outside of the known genes' boundaries is limited. Here, we use high-throughput transcriptome sequencing (RNA-Seq) to characterize the total non-ribosomal transcriptome of human, chimpanzee, and rhesus macaque brain. In all species, only 20-28% of non-ribosomal transcripts correspond to annotated exons and 20-23% to introns. By contrast, transcripts originating within intronic and intergenic repetitive sequences constitute 40-48% of the total brain transcriptome. Notably, some repeat families show elevated transcription. In non-repetitive intergenic regions, we identify and characterize 1,093 distinct regions highly expressed in the human brain. These regions are conserved at the RNA expression level across primates studied and at the DNA sequence level across mammals. A large proportion of these transcripts (20%) represents 3'UTR extensions of known genes and may play roles in alternative microRNA-directed regulation. Finally, we show that while transcriptome divergence between species increases with evolutionary time, intergenic transcripts show more expression differences among species and exons show less. Our results show that many yet uncharacterized evolutionary conserved transcripts exist in the human brain. Some of these transcripts may play roles in transcriptional regulation and contribute to evolution of human-specific phenotypic traits. |
| Keywords: | Cerebellum, Chromosome Mapping, Cluster Analysis, Intergenic DNA, Gene Expression Profiling, Macaca, Genetic Models, Pan Troglodytes, Phenotype, Nucleic Acid Repetitive Sequences, Sequence Alignment, RNA Sequence Analysis, Species Specificity, Animals, Mice |
| Source: | PLoS Computational Biology |
| ISSN: | 1553-734X |
| Publisher: | Public Library of Science |
| Volume: | 6 |
| Number: | 7 |
| Page Range: | e1000843 |
| Date: | 1 July 2010 |
| Official Publication: | https://doi.org/10.1371/journal.pcbi.1000843 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
