The landscape of C. elegans 3'UTRs

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Item Type:	Article
Title:	The landscape of C. elegans 3'UTRs
Creators Name:	Mangone, M., Manoharan, A.P., Thierry-Mieg, D., Thierry-Mieg, J., Han, T., Mackowiak, S., Mis, E., Zegar, C., Gutwein, M.R., Khivansara, V., Attie, O., Chen, K., Salehi-Ashtiani, K., Vidal, M., Harkins, T.T., Bouffard, P., Suzuki, Y., Sugano, S., Kohara, Y., Rajewsky, N., Piano, F., Gunsalus, K.C. and Kim, J.K.
Abstract:	Three-prime untranslated regions (3'UTRs) of metazoan mRNAs contain numerous regulatory elements, yet remain largely uncharacterized. Using polyA capture, 3'RACE, full-length cDNAs, and RNA-seq, we define ~26,000 distinct 3'UTRs in Caenorhabditis elegans for ~85% of the 18,328 experimentally supported protein coding genes and revise ~40% of gene models. Alternative 3'UTR isoforms are frequent, often differentially expressed during development. Average 3'UTR length decreases with animal age. Surprisingly, no polyadenylation signal (PAS) is detected for 13% of polyA sites, predominantly among shorter alternative isoforms. Trans-spliced (vs. non-trans-spliced) mRNAs possess longer 3'UTRs and frequently contain no PAS or variant PAS. We identify conserved 3'UTR motifs, isoform-specific predicted microRNA target sites, and polyadenylation of most histone genes. Our data reveal a rich complexity of 3'UTRs genome-wide and throughout development.
Keywords:	Binding Sites, Computational Biology, Conserved Sequence, Developmental Gene Expression Regulation, Gene Library, Helminth Genes, Helminth Proteins, Helminth RNA, Hermaphroditism, Histones, Messenger RNA, MicroRNA, Operon, Poly A, Polyadenylation, Trans-Splicing, Animals, Caenorhabditis elegans
Source:	Science
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Volume:	329
Number:	5990
Page Range:	432-435
Date:	23 July 2010
Official Publication:	https://doi.org/10.1126/science.1191244
PubMed:	View item in PubMed

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