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Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Item Type:Article
Title:Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
Creators Name:Izsvak, Z., Froehlich, J., Grabundzija, I., Shirley, J.R., Powell, H.M., Chapman, K.M., Ivics, Z. and Hamra, F.K.
Abstract:Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.
Keywords:DNA Transposable Elements, Gene Knockout Techniques, Mutagenesis, Spermatogonia, Stem Cells, Animals, Rats
Source:Nature Methods
ISSN:1548-7091
Publisher:Nature Publishing Group
Volume:7
Number:6
Page Range:443-445
Date:June 2010
Official Publication:https://doi.org/10.1038/nmeth.1461
PubMed:View item in PubMed

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