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| Item Type: | Article |
|---|---|
| Title: | Intratumoral low-volume jet-injection for efficient nonviral gene transfer |
| Creators Name: | Walther, W., Stein, U., Fichtner, I., Voss, C., Schmidt, T., Schleef, M., Nellessen, T. and Schlag, P.M. |
| Abstract: | Jet-injection has become an applicable technology among other established nonviral delivery systems, such as particle bombardment or in vivo electroporation. The low-volume jet injector employed in this study uses compressed air to inject solutions of 1.5-10 {my}L containing naked DNA into the desired tissue. The novel design of this prototype makes multiple jet-injections possible. Therefore, repeated jet-injections into one target tissue can be performed easily. This jet-injector hand-held system was used for the direct in vivo gene transfer of plasmid DNA into tumors to achieve efficient expression of reporter genes ({beta}-galactosidase, green fluorescent protein [GFP]) and of therapeutic genes (TNF-{alpha}) in different tumor models. The study presented here revealed the key parameters of efficient in vivo jet-injection (jet-injection volume, pressure, jet penetration, DNA stability) to define the optimal conditions for a jet-injection-aided nonviral gene therapy. |
| Keywords: | Gene Therapy, Jet-Injection, Naked DNA, Nonviral Gene Transfer, Plasmid Stability, Plasmid Topology |
| Source: | Molecular Biotechnology |
| ISSN: | 1073-6085 |
| Publisher: | Humana Press |
| Volume: | 21 |
| Number: | 2 |
| Page Range: | 105-115 |
| Date: | 1 June 2002 |
| Official Publication: | https://doi.org/10.1385/MB:21:2:105 |
| PubMed: | View item in PubMed |
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