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Global transcriptomic analysis of murine embryonic stem cell-derived brachyury+ (T) cells

Item Type:Article
Title:Global transcriptomic analysis of murine embryonic stem cell-derived brachyury+ (T) cells
Creators Name:Doss, M.X., Wagh, V., Schulz, H., Kull, M., Kolde, R., Pfannkuche, K., Nolden, T., Himmelbauer, H., Vilo, J., Hescheler, J. and Sachinidis, A.
Abstract:Brachyury+ mesodermal cell population with purity over 79% was obtained from differentiating brachyury embryonic stem cells (ESC) generated with brachyury promoter driven enhanced green fluorescent protein and puromycin-N-acetyltransferase. A comprehensive transcriptomic analysis of brachyury+ cells enriched with puromycin application from 6-day-old embryoid bodies (EBs), 6-day-old control EBs and undifferentiated ESCs led to identification of 1573 uniquely up-regulated and 1549 uniquely down-regulated transcripts in brachyury+ cells. Furthermore, transcripts up-regulated in brachyury+ cells have overrepresented the Gene Ontology annotations (cell differentiation, blood vessel morphogenesis, striated muscle development, placenta development and cell motility) and Kyoto Encyclopedia of Genes and Genomes pathway annotations (mitogen-activated protein kinase signaling and transforming growth factor beta signaling). Transcripts representing Larp2 and Ankrd34b are notably up-regulated in brachyury+ cells. Knockdown of Larp2 resulted in a significantly down-regulation BMP-2 expression, and knockdown of Ankrd34b resulted in alteration of NF-H, PPAR gamma and PECAM1 expression. The elucidation of transcriptomic signatures of ESCs-derived brachyury+ cells will contribute toward defining the genetic and cellular identities of presumptive mesodermal cells. Furthermore, there is a possible involvement of Larp2 in the regulation of the late mesodermal marker BMP-2. Ankrd34b might be a positive regulator of neurogenesis and a negative regulator of adipogenesis.
Keywords:Activated Receptor alpha, Mesoderm Formation, In Vitro, Gene, Expression, Protein, Differentiation, Commitment, Database, Animals, Xenopus
Source:Genes to Cells
Page Range:209-228
Date:March 2010
Official Publication:https://doi.org/10.1111/j.1365-2443.2010.01390.x

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